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Long PCR Protocol
37714
Long PCR Protocol
http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.html
Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA
Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
Jan 6, 2008
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Other links at Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
Article on the influence of the design of the primer on the outcome of PCR, by Dr Vincent R. Prezioso, published by Eppendorf, North America.
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2. PCR Protocol
Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.
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Preparation of DNA from plant tissues suitable for PCR methods including AFLP, article by DH CHEN and PC RONALD Department of Plant Pathology, University of California, Davis.
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Basic discussion of the optimization of PCR, from the web site of Brinkmann Instruments.
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From Molecular Biology Techniques Manual, from the web site of the University of Cape Town, South Africa.
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From the Jackson Laboratory, University of Maine, USA.
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An introduction by Connie Veilleux from the US National Health Museum website.
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Popular survey article by Mark V. Bloom, DNA Learning Center, Cold Spring Harbor Laboratory, from the web site of the US National Health Museum.
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9. PCR Gateway
A directory of PCR techniques, PCR protocols, PCR troubleshooting, PCR websites and online resources from the publisher Horizon Press.
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Protocols including standard nested PCR, from the Biotechnology Laboratory, University of British Columbia.
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