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PCR Protocols
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PCR Protocols
http://www.cas.psu.edu/docs/CASDEPT/VET/jackvh/jvhpcr.html
Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State University
Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
Jan 6, 2008
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Other links at Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
Bacteria are characterized by Rep-PCR fingerprinting using primers corresponding to naturally occurring repetitive sequences in the interspersed regions.
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Protocol for Randomly Amplified Polymorphic DNA, by H Judelson, University of California, Riverside, USA.
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The identification of novel members of gene families by PCR using degenerate primers is described and protocols given. Article by Michael Koelle 1996 on the web site of Dartmouth College.
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Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA
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Tests on gruup B Streptococcus and methicillin resistant Staphylococcus aureus by PCR / DNA sequencing.
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6. RAPD's
Brief description of Random Amplified Polymorphic DNA, from Oklahoma State University, USA.
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Protocols including standard nested PCR, from the Biotechnology Laboratory, University of British Columbia.
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Article by PN Hengen from TIBS 1995
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9. Kary Mullis
Inventor Profile of Kary Mullis, the originator of PCR, from the National Inventors Hall of Fame web site.
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An animation explaining how the Polymerase Chain Reaction (PCR) works, from the Dolan DNA learning center, Cold Spring Harbor Laboratory, USA.
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