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Detection of Asymmetric PCR Products in Homogeneous Solution by Fluorescence Correlation Spectroscopy
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Detection of Asymmetric PCR Products in Homogeneous Solution by Fluorescence Correlation Spectroscopy
http://imd-www.es.hokudai.ac.jp/~kinjo/fapcr/fapcrout.html
In asymmetric PCR, the low concentration primer is quantitatively incorporated into double stranded DNA after an appropriate number of cycles. If this primer is fluorescent labelled, the dsDNA can be quantitated from the fluorescence.
Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
Jan 6, 2008
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Other links at Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
Preparation of DNA from plant tissues suitable for PCR methods including AFLP, article by DH CHEN and PC RONALD Department of Plant Pathology, University of California, Davis.
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This method works because the poison allows the formation of deletion products but titers out full-sized products. From the Biotechnology Laboratory, University of British Columbia.
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, European Union DGXII Biotechnology FW IV Research Programme. From the web site of the University Library, GA¶ttingen.
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Article in which probes from different libraries were used to hybridize seven cereals at the Department of Plant Breeding and Biometry, Cornell University, NY.
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Protocol for RAP-PCR to study prokaryotic gene expression, from the Enterococcus Research Site, University of Oklahoma, USA.
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6. RAPD PCR
RAPD stands for Random Amplification of Polymorphic DNA, where the target sequence(s) (to be amplified) is unknown.Brief description, from Rutgers University, USA.
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7. HiFi DNA
HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails.
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8. PCR Guru
A downloadable textbook on PCR setup and optimization, not free.
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Bacteria are characterized by Rep-PCR fingerprinting using primers corresponding to naturally occurring repetitive sequences in the interspersed regions.
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10. PCR Technology
An introduction by Connie Veilleux from the US National Health Museum website.
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