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Standard PCR protocols
37737
Standard PCR protocols
http://web.uct.ac.za/microbiology/pcrcond.htm
From Molecular Biology Techniques Manual, from the web site of the University of Cape Town, South Africa.
Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
Jan 6, 2008
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Other links at Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
1. Kary Mullis
Inventor Profile of Kary Mullis, the originator of PCR, from the National Inventors Hall of Fame web site.
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Popular description of the PCR technique by John C Brown, University of Kansas 1995.
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3. PCR Protocol
Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.
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Brief guidelines. From the Jackson Laboratory, University of Maine, USA.
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Intended for specialists planning PCR procedures in their laboratories. From the University of Cape Town updated 2001.
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Examples of Restriction Fragment Length Polymorphism (RFLP)electrophoresis slabs for different nematodes, from University of Nebrasca.
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7. PCR Guru
A downloadable textbook on PCR setup and optimization, not free.
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In asymmetric PCR, the low concentration primer is quantitatively incorporated into double stranded DNA after an appropriate number of cycles. If this primer is fluorescent labelled, the dsDNA can be quantitated from the fluorescence.
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Tests on gruup B Streptococcus and methicillin resistant Staphylococcus aureus by PCR / DNA sequencing.
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A report and protocol, from Fondation Jean Dausset.
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