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PCR Protocol
37740
PCR Protocol
http://www.biology.lsa.umich.edu/research/labs/maddock/protocols/PCR/general_pcr_protocol.html
Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA.
Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
Jan 6, 2008
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Other links at Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
Article by Ed Rybicki, Department of Molecular and Cell Biology, University of Cape Town in: Molecular Biology Techniques Manual, on the web site of the University of Cape Town.
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2. HiFi DNA
HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails.
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C, Inc Bridgewater, NJ, USA.
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From Molecular Biology Techniques Manual, from the web site of the University of Cape Town, South Africa.
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Applications in work on aging of Caenorhabditis elegans and phylogeny of nematodes, by Andy Vierstraete, Department of Biology, University of Ghent, Belgium.
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Popular survey article by Mark V. Bloom, DNA Learning Center, Cold Spring Harbor Laboratory, from the web site of the US National Health Museum.
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7. PCR Guru
A downloadable textbook on PCR setup and optimization, not free.
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P Martins-Lopez, H Zhang, R Koebner, Plant Mol. Biol. Reporter 19(2001): 159-162. From National Research Coouncil Canada.
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Article in which probes from different libraries were used to hybridize seven cereals at the Department of Plant Breeding and Biometry, Cornell University, NY.
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A page describing the main parameters and trouble-shooting in PCR. The page is somewhat dated (updated 1997) but still useful.
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