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Quantitative PCR Protocol
37760
Quantitative PCR Protocol
http://www.jax.org/cyto/quanpcr.html
From the Jackson Laboratory, University of Maine, USA.
Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
jackson laboratory opportunities labs jaxmice laboratories mice bar harbor maine mouse jax research resources scientific mammalian genetic disease genome jax.org cytogenetics down syndrome protocols muriel davisson
Jan 6, 2008
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Other links at Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
RFLP = Restriction Fragment Length Polymorphism. By Alexander Binder 1997.
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A page describing the main parameters and trouble-shooting in PCR. The page is somewhat dated (updated 1997) but still useful.
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An introduction with principle, applications and description of the technique. From the web site of Kebangsaan University, Malaysia.
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Division of BioRad Laboratories that manufactures and sells instruments for PCR, in Hercules, California, USA.
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In asymmetric PCR, the low concentration primer is quantitatively incorporated into double stranded DNA after an appropriate number of cycles. If this primer is fluorescent labelled, the dsDNA can be quantitated from the fluorescence.
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Technical aspects of quantitative real-time PCR and RT-PCR. Instruments, kits, dyes, chemistries, and services presented by their manufacturers.
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Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240.
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Discussion of their origin and briefly their properties. From the web site of Colorado State University.
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Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK.
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P Martins-Lopez, H Zhang, R Koebner, Plant Mol. Biol. Reporter 19(2001): 159-162. From National Research Coouncil Canada.
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