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Optimizing PCR Protocols
37761
Optimizing PCR Protocols
http://www.jax.org/imr/optimize_pcr.html
Brief guidelines. From the Jackson Laboratory, University of Maine, USA.
Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
jackson laboratory opportunities labs jaxmice laboratories mice bar harbor maine mouse jax research resources scientific mammalian genetic disease genome jax.org
Jan 6, 2008
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Other links at Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
1. PCR Gateway
A directory of PCR techniques, PCR protocols, PCR troubleshooting, PCR websites and online resources from the publisher Horizon Press.
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2. Kary Mullis
Inventor Profile of Kary Mullis, the originator of PCR, from the National Inventors Hall of Fame web site.
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step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.
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A graphic description of the principle of PCR from the US National Health Museum web site.
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Discussion of their origin and briefly their properties. From the web site of Colorado State University.
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Application of inverse PCR, partial genomic libraries and TAIL-PCR in cloning flanking, at the Department of Biological Sciences, Dartmouth College, Hanover, NH.
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. Article by PN Hengen in TIBS June 1997.
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Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA.
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9. HiFi DNA
HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails.
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10. RAPD PCR
RAPD stands for Random Amplification of Polymorphic DNA, where the target sequence(s) (to be amplified) is unknown.Brief description, from Rutgers University, USA.
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