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Degenerate PCR
37767
Degenerate PCR
http://www.dartmouth.edu/~ambros/protocols/other/koelle/degenerate_PCR.html
The identification of novel members of gene families by PCR using degenerate primers is described and protocols given. Article by Michael Koelle 1996 on the web site of Dartmouth College.
Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
Jan 6, 2008
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Other links at Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
The identification of novel members of gene families by PCR using degenerate primers is described and protocols given. Article by Michael Koelle 1996 on the web site of Dartmouth College.
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Division of BioRad Laboratories that manufactures and sells instruments for PCR, in Hercules, California, USA.
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A report and protocol, from Fondation Jean Dausset.
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Article in which probes from different libraries were used to hybridize seven cereals at the Department of Plant Breeding and Biometry, Cornell University, NY.
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Limited to conventional straight forward PCR. Page designed and maintained by Octavian Henegariu on the web site of the Yale - New Haven Medical Center.
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6. RAPD's
Brief description of Random Amplified Polymorphic DNA, from Oklahoma State University, USA.
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Brief discussion of additives to improve amplification efficiency and specificity of PCR, by Octavian Henegariu, Yale-New Haven Medical Center.
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Article by PN Hengen from TIBS 1995
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step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.
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Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK.
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