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Detection of Point Mutations by RFLP of PCR Amplified DNA Sequences
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Detection of Point Mutations by RFLP of PCR Amplified DNA Sequences
http://www.uni-graz.at/~binder/thesis/node64.html
RFLP = Restriction Fragment Length Polymorphism. By Alexander Binder 1997.
Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
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Jan 6, 2008
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Other links at Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
Article by PN Hengen from TIBS 1995
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Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA.
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Popular description of the PCR technique by John C Brown, University of Kansas 1995.
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Article on the influence of the design of the primer on the outcome of PCR, by Dr Vincent R. Prezioso, published by Eppendorf, North America.
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The identification of novel members of gene families by PCR using degenerate primers is described and protocols given. Article by Michael Koelle 1996 on the web site of Dartmouth College.
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56 page booklet on oligonucleotides, PCR and RT-PCR, from Biosource (company).
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Protocol by M McMurray, Fred Hutchinson Cancer Research Center, Seattle, Wa. USA.
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This method works because the poison allows the formation of deletion products but titers out full-sized products. From the Biotechnology Laboratory, University of British Columbia.
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9. RAPD PCR
RAPD stands for Random Amplification of Polymorphic DNA, where the target sequence(s) (to be amplified) is unknown.Brief description, from Rutgers University, USA.
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10. PCR Gateway
A directory of PCR techniques, PCR protocols, PCR troubleshooting, PCR websites and online resources from the publisher Horizon Press.
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