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C. elegans Gene Knockout Project
37779
C. elegans Gene Knockout Project
http://ko.cigenomics.bc.ca/
Protocols including standard nested PCR, from the Biotechnology Laboratory, University of British Columbia.
Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
Jan 6, 2008
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Other links at Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
1. GenHunter
Manufacturer of material for differential display PCR in Nashville, Tenn USA.
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2. RAPD's
Brief description of Random Amplified Polymorphic DNA, from Oklahoma State University, USA.
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Extensive collection of PCR protocols and methods from Protocol On Line.
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Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA.
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Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA
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Limited to conventional straight forward PCR. Page designed and maintained by Octavian Henegariu on the web site of the Yale - New Haven Medical Center.
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7. PCR Guru
A downloadable textbook on PCR setup and optimization, not free.
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Article by PN Hengen from TIBS 1995 on the loss of activity of PCR primers with time.
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Intended for specialists planning PCR procedures in their laboratories. From the University of Cape Town updated 2001.
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10. Reference in PCR
Technical aspects of quantitative real-time PCR and RT-PCR. Instruments, kits, dyes, chemistries, and services presented by their manufacturers.
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