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General Notes on Primer Design in PCR
37781
General Notes on Primer Design in PCR
http://www.eppendorfna.com/applications/pcr_appl_primer.asp?SX=NF&
Article on the influence of the design of the primer on the outcome of PCR, by Dr Vincent R. Prezioso, published by Eppendorf, North America.
Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
bio tools pipettes pcr molecular technologies eppendorf applications application protocols bionews award neuroscience biotechnology life science thermomixers thermostat titration dispenser buret pipette tips pipet
Jan 6, 2008
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Other links at Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR
Brief discussion of additives to improve amplification efficiency and specificity of PCR, by Octavian Henegariu, Yale-New Haven Medical Center.
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A report and protocol, from Fondation Jean Dausset.
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3. PCR Gateway
A directory of PCR techniques, PCR protocols, PCR troubleshooting, PCR websites and online resources from the publisher Horizon Press.
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Extensive collection of PCR protocols and methods from Protocol On Line.
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In asymmetric PCR, the low concentration primer is quantitatively incorporated into double stranded DNA after an appropriate number of cycles. If this primer is fluorescent labelled, the dsDNA can be quantitated from the fluorescence.
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Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA.
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step by step protocol, from the web site of the Department of Biology, University of Michigan, USA.
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An introduction by Connie Veilleux from the US National Health Museum website.
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The originator of PCR, from the Nobel e-museum web site.
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10. RAPD's
Brief description of Random Amplified Polymorphic DNA, from Oklahoma State University, USA.
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