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PCR Guru

37782 PCR Guru http://www.pcrguru.com/ A downloadable textbook on PCR setup and optimization, not free. Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR pcr   polymerase   chain   reaction   optimization   troubleshooting   magnesium   titration   dntp   tm   laboratory   dna   rna   gene   vector   genome   bacteria   virus   cell   cloning   experiment   trick   method   biotechnology   genetics   molecular   biology   ebook   ebo Jan 6, 2008  

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Other links at Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR

An introduction with principle, applications and description of the technique. From the web site of Kebangsaan University, Malaysia.
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Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK.
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Applications in work on aging of Caenorhabditis elegans and phylogeny of nematodes, by Andy Vierstraete, Department of Biology, University of Ghent, Belgium.
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RFLP = Restriction Fragment Length Polymorphism. By Alexander Binder 1997.
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For characterization colonies of transformed clones of Saccharaomyces, from the web site of the Stanford Genome Technology Center, Palo Alto, CA, USA.
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A report and protocol, from Fondation Jean Dausset.
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Technical aspects of quantitative real-time PCR and RT-PCR. Instruments, kits, dyes, chemistries, and services presented by their manufacturers.
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Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA
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Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240.
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Article by PN Hengen from TIBS 1995
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