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Differential Display PCR
37799
Differential Display PCR
http://www.genome.iastate.edu/edu/newtech/ddpcr.html
Used to study differences in gene expression using RT PCR. From: U.S. National Animal Genome Research Project.
Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR > RNA to DNA Amplification
animal livestock pig swine chicken sheep cattle sow boar pork veal cow bull ewe lamb porcine bovine ovine genome mapping gene map dna molecular genomics database genetics discussion nagrp national research progra
Jan 6, 2008
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Other links at Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR > RNA to DNA Amplification
Detailed protocol, based on P Song, E Yamamoto, R Allen: Plant Mol. Biol. Rep. 13(1995):174-181. From the web site of the GrainGenes ACEDB Database.
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Used to study differences in gene expression using RT PCR. From: U.S. National Animal Genome Research Project.
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3. Improvements in quantitative real-time RT-PCR with QIAGEN Omniscript and Sensiscript RTs (PDF file)
Evalutaion of two sets of reagents for Real Time Reverse Transcriptase PCR to determine gene expression. By S Ullmann et al.,QIAGEN GmbH, Hilden, Germany;.
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Description of the technique and listing of materials supplied by GenHunter, in Nashville, TN, USA.
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Survey article: J Sturtevant: Clin. Microbiol. Rev. 2000: 13(3) 408-427.
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6. Effect of Primer Purity on the Banding Patterns of Differential Display Polymerase Chain Reaction
Article: L Wen: J Biomol Tech 2000;11:87-91, San Diego State University, CA, USA.
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Lab protocol by Liu at Plant Research International, Wageningen, The Netherlands.
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Article: S Lievens, S Goormachtig, M Holsters: Nuc Acids Res. 2001: 29(17) 3459-3468.
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Animation to illustrate the way reverse transcriptase PCR (RT-PCR) produces DNA copies of an RNA template, from Cold Spring Harbor Laboratory Learning Center, New York.
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