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Ammonium sulfate fractionation of an extract from the leaves of spinach (Spinacia oleracea L.) produced two fractions of phosphofructokinase activity, the first stimulated by inorganic phosphate and the second inhibited by inorganic phosphate.
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Under the conditions specified, the world record rate.(which still holds) of light-induced ATP forma- tion, approaching 2500 µ moles X mg chl-1 X hr-1 , was attained.
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