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NADP-dependent isocitrate dehydrogenase was purified from Brassica oleracea (cauliflower) using ammonium sulfate precipitation, Matrex Red A affinity chromatography and Sephacryl S-300 gel filtration.
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Dark-grown carrot (Daucus carota L.) tissue cultures were found to contain both protein components of the NADP/thioredoxin system--NADP-thioredoxin reductase and the thioredoxin characteristic of heterotrophic systems, thioredoxin h.
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Constitutive expression of a celery mannitol dehydrogenase in tobacco enhances resistance to the mannitol-secreting fungal pathogen Alternaria alternata Dianne B. Jennings1,y , Margaret E. Daub2 , D. Mason Pharr1 and John D.
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About three hundred spots were detected on the 2-DE map after colloidal blue staining. From 2-DE map of cv. Gamay mesocarp, 67 proteins were identified (p . 0.95) using matrix-assisted laser desorption/ioniza- tion-mass spectrometry analysis.
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