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By knocking out a gene, researchers are better able to study the function of the inactivated gene. One way to accomplish this inactivation is by using a knockout vector. Knockout plants can aid scientists in a variety of areas, including disease resistance.
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Dihydroflavonol reductase (DFR) is a key enzyme involved in anthocyanin biosynthesis and proanthocyanidin synthesis in grape. DFR catalyses the reduction of dihydroflavonols to leucoanthocyanidins in the anthocyanin pathway.
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Here, a novel polymerase chain reaction (PCR) based RNAi vector pTCK303 with a maize ubiquitin promoter, 2 specific multiple enzyme sites, and a rice intron was constructed for monocot gene silencing.
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