promoter, which is known to be one of the most potent promoters in mammalian cells. The 35S promoter must therefore be considered to be a promoter of significant potency in mammalian cells.
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(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.) Click on image to view larger version.
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The presented work integrates molecular data on gene expression with anatomical and biochemical data to analyze the development and the sucrose accumulation process in sugar beet (Beta vulgaris L.) roots.
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bstract. Quantitative real-time PCR (RT-PCR) has already been used to study the expression profile of several plant gene families (Yokoyama and Nishitani, 2001; Mladek et al., 2003; Charrier et al., 2002).
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Offers restriction endonucleases, recombinant enzymes and reagents for chemiluminscence labeling, RNA inference, DNA synthesis, sequencing and mutagenesis. International contacts, with headquarters in Ipswich, MD, USA.
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List of links and forum on the subject and related methodology. Set up and maintained by SJ Krivokapich, National University of Misiones, Argentina.
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A page describing the main parameters and trouble-shooting in PCR. The page is somewhat dated (updated 1997) but still useful.
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Technical aspects of quantitative real-time PCR and RT-PCR. Instruments, kits, dyes, chemistries, and services presented by their manufacturers.
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Database of validated primer sets and other resources for quantitative real time PCR.
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News- and overview site on technical aspects of gene expression analysis with real-time RT-PCR and competitive RT-PCR from the Technical University of Munich, Germany
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RNA and DNA isolation reagents, oligo dT columns, hybridization reagents and other molecular biology reagents designed by P. Chomczynski, Cincinnati, OH.
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Reagents and kits for genomics, proteomics, and RNA research from Madison, Wisconsin.
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