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MS/MS with multiple reaction monitoring (MRM) detection was carried out to obtain sufficient selectivity and sensitivity for the cytokinin nucleotides. The combined use of on-line sample stacking and CZE-MS/MS achieved limits of detection (LODs) in the range of 0.06-0.
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Pressurized liquid extraction (PLE) at room temperature with a laboratory-assembled system was applied for the extraction of gastrodin (GA) and vanillyl alcohol (VA) in Gastrodia elata Blume.
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