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We have established a regeneration protocol for melon (Cucumis melo L.), using cotyledons as explants, in which the overall regeneration frequency reached more than 90 percent, and combined this protocol with Agrobacterium tumefaciens transformation to produce transgenic melon plants.
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The genetic engineering community has assumed that Agrobacterium, a commonly used gene transfer vector for plants, does not infect animal cells, and certainly would not transfer genes into them. But this has been proved wrong. Prof. Joe Cummins warns of hazards to laboratory and farm workers.
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15. 'Ulu (Breadfruit; Artocarpus altilis): Pacific Islands, probably Guam; Root sprouts (dig up small plants growing from large tree. Leave 6 inches of root or more on each side); Food, Craft, etc. 16.
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